強大高效，適合大規?；蚪M學
| HISEQ SBS V4 PRE-RELEASE SPECIFICATIONS | TRUSEQ SBS V3 | |||||
|---|---|---|---|---|---|---|
| Read length | Dual Flow Cell | Single Flow Cell | Dual Flow Cell Run Time | Dual Flow Cell | Single Flow Cell | Dual Flow Cell Run Time |
| 1×36 | 128-144 Gb | 64-72 Gb | 29 hrs | 95-105 Gb | 47-52 Gb | 2 days |
| 2×50 | 360-400 Gb | 180-200 Gb | 2.5 days | 270-300 Gb | 135-150 Gb | 5.5 days |
| 2×100 | 720-800 Gb | 360-400 Gb | 5 days | 540-600 Gb | 270-300 Gb | 11 days |
| 2×125 | 900-1 Tb | 450-500 Gb | 6 days | N/A | N/A | N/A |
| Reads Passing Filter | Up to 4 billion single read or 8 billion paired-end reads | Up to 2 billion single read or 4 billion paired-end reads | Up to 3 billion single read or 6 billion paired-end reads | Up to 1.5 billion single read or 3 billion paired-end reads | ||
| Quality |
Greater than 85% of bases above Q30 at 2×50 bp Greater than 80% of bases above Q30 at 2×100 bp Greater than 80% of bases above Q30 at 2×125 bp |
Greater than 85% of bases above Q30 at 2×50 bp Greater than 80% of bases above Q30 at 2×100 bp |
||||
*Install specifications based on Illumina PhiX control library at supported cluster densities (between 610-678 K clusters/mm2 passing filter using TruSeq v3 Kits or 950-1050 K clusters/mm2 passing filter using HiSeq v4). Run times high output mode correspond to sequencing only. Performance may vary based on sample quality, cluster density, and other experimental factors.
| Read length | Dual Flow Cell | Single Flow Cell | Dual Flow Cell Run Time |
| 1×36 | 18-22 Gb | 9-11 Gb | 7 hr |
| 2×50 | 50-60 Gb | 25-30 Gb | 16 hr |
| 2×100 | 100-120 Gb | 50-60 Gb | 27 hr |
| 2×150 | 150-180 Gb | 75-90 Gb | 40 hr |
| Reads Passing Filter | Up to 600 million single read or 1.2 billion paired-end reads | Up to 300 million single read or 600 million paired-end reads | |
| Quality |
Greater than 85% of bases above Q30 at 2×50 bp Greater than 80% of bases above Q30 at 2×100 bp Greater than 75% of bases above Q30 at 2×150 bp |
||
讓應用更輕松，讓您專注于研究
從 樣品制備到數據分析，我們的重點是讓應用更輕松 – 這樣您的重點就能放在您的研究上。靶向重測序、基因表達、全基因組測序、表觀遺傳學，以及更多 – 我們設計了完整的解決方案，以簡化您想要開展的任何研究。無論您的研究目標、實驗室規?；蚪涃M如何，HiSeq 2500系統都為您所有的高通量測序需求提供了一個理想的解決方案。
全基因組測序就在您的實驗室
在 您的實驗室里就能快速經濟地開展基因組測序。選擇HiSeq 2500系統的快速運行模式，加速周轉時間，在一天內測序整個人類基因組?；蛘?，保持高產量模式，在單次運行中測序多個基因組，假定每個基因組為100 Gb。兩種模式都產生了較好的數據質量，帶來準確、完整的全基因組測序結果。
靶向重測序
從單個定制panel到整個外顯子組，從一整套靶向重測序方案中選擇，以支持所有研究設計。經濟高效且可擴展的探索、驗證和篩查如今觸手可及。
全轉錄組測序
從高通量的基因表達譜分析到深度的轉錄分析，HiSeq提供了前所未有的RNA分析方案。測定單個轉錄本和亞型的豐度，發現新的基因，并鑒定非編碼調控RNA。
De Novo 測序
有了高產量、長reads和雙端測序的獨特組合，HiSeq成為任何大小基因組de novo測序的強大工具。通過測序整個微生物群落，開展真正的宏基因組分析，以發現重要的分類和功能信息。
表觀遺傳調控
在HiSeq單次運行中，通過評估多種形式的表觀遺傳調控，了解更加完整的生物學故事。利用ChIP-Seq研究DNA與蛋白的互作和基因調控。以單堿基分辨率定量DNA甲基化。